[카테고리:] Southern/Nothern Blot analysis

14개 결과 출력

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  [BC003] CHURCH Buffer

  Cat. No. – BC003

  Package – 1L

  Storage – RT

  Description – 1% BSA, 1mM EDTA, 0.5M Na2HPO4, pH7.2, 7.0% SDS

  Application – For use in DNA and RNA hybridization applications

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  [BH001] Hybridization Solution I

  Cat. No. –BH001

  Package –1000ml

  Storage –RT

  Description –0.9M NaCl, 90mM Trisodium citrate with 5.0%Tween 20

  Application –Ready-to-use hybidization solution optimized for in situ hybidization (ISH) procedures

  * For R&D use only

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  [BH006] Hybridization Solution w/formamide

  Cat. No. –BH006

  Package –500ml

  Storage –RT

  Description –40mM PIPES,pH 6.8, 1m M EDTA, pH 8.0, 0.4M NaCl, 80%(v/v) Deionized formamide

  Application –Used for detection of biotinylated probes on membranes in procedures such as Northern and Southern Blotting,and dot blots

  * For R&D use only

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  [BH007] Hybridization Solution w/o formamide

  Cat. No. – BH007

  Package –500ml

  Storage –RT

  Description –40mM PIPES,pH 6.4, 0.1mM EDTA, pH 8.0, 0.4M NaCl

  Application –Suitable for Southern, Northern and Dot Blot hybridization analysis, hybridization with oligonucleotides or other types of probes, and for hybridization screening of  gene libraries

  * For R&D use only

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  [BM003] 10X MOPS Buffer

  Cat. No. – BM003

  Package – 1,000ml

  Storage – Room Temperature.

  Description – 200mM MOPS free acid, 50mM sodium acetate, 10mM EDTA, and 10mM EGTA. pH 7.0, Treated with DEPC

  Application – Used as the electrophoresis buffer for formaldehyde (denaturing) agarose gels

  * For R&D use only

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  [BN020] Neutralization Buffer for Southern blot

  Cat. No. – BN020

  Package – 1000ml

  Storage – RT

  Description – Tris-HCl, pH 7.5 0.5M, Sodium chloride 1.5M

  Application – Suitable for Southern blot analysis and other molecular biology application.

  * For R&D use only

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  [BP032] Prehybridization / Hybridization Solution

  Cat. No. – BP032

  Package – 500ml

  Storage – RT

  Description – Sodium phosphate pH 7.2 0.5M, SDS 7%(w/v)
  

  Application – Suitable for use as a pre-hybridization buffer in Northern and Southern blot protocols

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  [BS011] 20X SSC

  Cat. No. – BS011

  Package – 1,000ml
   
  Storage – RT

  Description – Sodium chloride 3M, Trisodium citrate 300mM, filtered solution

  Application – For use in nucleic acid hybridizations and blot transfer applications

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  [BS012] 20X SSP

  Cat. No. – BS012

  Package – 1,000ml
   
  Storage – RT

  Description – Sodium chloride 3M, Sodium phosphate (monobasic anhydrous) 200mM pH 7.4

  Application – For use in nucleic acid hybridizations and blot transfer applications

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  [BS013a] 20X SSPE

  Cat. No. BS013a

  Package -500ml

  Storage – RT

  Application – For use in nucleic acid hybridizations and blot transfer applications

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  [BS013] 20X SSPE

  Cat. No. – BS013

  Package – 1,000ml
   
  Storage – RT

  Description – Sodium chloride 3M, Sodium phosphate (monobasic anhydrous) 200mM, EDTA 20mM pH 7.4

  Application – For use in nucleic acid hybridizations and blot transfer applications

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  [BW001] 10X Washing Buffer, pH 7.5

  Cat. No. –BW001

  Package –1000ml
   
  Storage – RT

  Description –Maleic acid 1M, NaCl 1.5M, pH 7.5 Solution
  pH adjusted with Sodium hydroxide (NaOH)
  10X Washing Buffer or 10X Maleic acid Buffer

  Application – Suitable for Sourthern and Northern blot analysis with DIG

  * For R&D use only

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  [BW004] Water, DEPC Treated

  Cat. No. –BW004

  Package –500ml
   
  Storage – RT

  Description –To treat solutions with DEPC, add 0.1% DEPC, mix and let it at room temperature O/N. Then autoclave.
  Purified by ion exchange, carbon extraction, glass distillation, and filtration. Resistance greater than 18.0M ohms.

  Application -Diethylpyrocarbonate (DEPC) treatment is the most commonly used method for eliminating RNase contamination from water, buffers, and other solutions.

  * For R&D use only

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  [OW004] Water, DEPC treated

  Cat. No. –OW004

  Package – 10L

  Storage – RT

  Description – To treat solutions with DEPC, add 0.1% DEPC, mix and let it at room temperature O/N. Then autoclave.

  Purified by ion exchange, carbon extraction, glass distillation, and filtration. Resistance greater than 18.0M ohms.

  Application – Diethylpyrocarbonate (DEPC) treatment is the most commonly used method for eliminating RNase contamination from water, buffers, and other solutions.

  * R&D use only

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