[SLP002] SAFELOAD 6X DNA-PAGE Loading Buffer
Cat. No. – SLP002
Package – 5ml
Storage – Cold (4-8℃) for 1 year, Avoid light
Description – red dye, non-carcinogen red fluorescent dye
Application – SAFELOAD Loading Buffer replace tixic Ethidium Bromide (EtBr)
Description :
SAFELOAD Loading Buffer replace toxic Ethidium Bromide (EtBr), a potent mutagen, for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose and polyacrylamide gel electrophoresis. SAFELOAD Loading Buffer contain red color tracking dye and a safe, non-carcinogen red fluorescent dye. Red dye is used for monitoring the agarose gel while it is running (approx 1 kb). During the gel electrophoresis, the agarose gel will reveal a red band. The gel can be directly visualized under UV light without any extra staining steps. DNA stained with the red florescent dye will emit a red light (595 nm), very similar to Ethidium Bromide (EtBr).
Protocol :
1. Mix your samples with the safeload Loading Buffer in a 1:5 ratio. For example, add 1μl of the safeload Loading Buffer to 5 μl of DNA sample; or 2 μl of the safeload Loading Buffer to 10 μl of DNA sample.
2. Run the agarose gel and visualize under LED or UV light when ready. Take picture as needed normally.
연관 상품
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[BM003] 10X MOPS Buffer
더 보기Cat. No. – BM003
Package – 1,000ml
Storage – Room Temperature.
Description – 200mM MOPS free acid, 50mM sodium acetate, 10mM EDTA, and 10mM EGTA. pH 7.0, Treated with DEPC
Application – Used as the electrophoresis buffer for formaldehyde (denaturing) agarose gels
* For R&D use only