[BP201] Paraformaldehyde-glutaraldehyde solution Kit
Cat. No. – BP201
Package – Kit
Storage – C
Using Paraformaldehyde-Glutaraldehyde Solution (Karnovsky’s Fixative)
Kit contains
- 2 x 10 ml 16% Paraformaldehyde Solution
- 1 x 10 ml 50% EM Grade Glutaraldehyde
- 1 x 50 ml Sodium Phosphate Buffer .2M, pH 7.2
Mixture 1 (Original Karnovsky’s Mixture)
| 16% Paraformaldehyde Solution | 20 ml |
| 50% Glutaraldehyde EM Grade | 8 ml |
| 0.2M Sodium Phosphate Buffer | 25 ml |
| Distilled Water | 25 ml |
Final mixture is 78 ml with 5% Glutaraldehyde, 4% Formaldehyde in 0.064M buffer. This mixture is extremely hypertonic, with an osmolarity of more than 2000m OSM .
Mixture 2
A lower concentraction of Glutaraldehyde and Formaldehyde especially used for vascular profusion.
| 16% Paraformaldehyde Solution | 13 ml |
| 50% Glutaraldehyde EM Grade | 5 ml |
| 0.2M Sodium Phosphate Buffer | 50 ml |
| Distilled Water | 32 ml |
Final mixture is 2% Paraformaldehyde, 2.5% Glutaraldehyde and 0.1M Buffer. If necessary, the osmolarity can be adjusted by adding sucrose to NaCl. The pH may also need to be adjusted to your desirable point. This mixture is the most effective general fixative for both plant and animal specimens.
연관 상품
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[BS018a] Stripping Buffer
더 보기Cat. No. –BS018a
Package –1L
Storage –15~30℃. Stable for a minimum of 1 years from date of receipt at room temperature
Description – 1X Stripping Buffer.
Application -Western Blot Stripping Buffer safely and effectively removes primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.
* For R&D use only