[SLP003] SAFELOAD 6X DNA-PAGE Loading Buffer
Cat. No. – SLP003
Package – 10 ml
Storage – Cold (4-8℃) for 1 year, Avoid light
Description – red dye, non-carcinogen red fluorescent dye
Application – SAFELOAD Loading Buffer replace tixic Ethidium Bromide (EtBr)
Description :
SAFELOAD Loading Buffer replace toxic Ethidium Bromide (EtBr), a potent mutagen, for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose and polyacrylamide gel electrophoresis. SAFELOAD Loading Buffer contain red color tracking dye and a safe, non-carcinogen red fluorescent dye. Red dye is used for monitoring the agarose gel while it is running (approx 1 kb). During the gel electrophoresis, the agarose gel will reveal a red band. The gel can be directly visualized under UV light without any extra staining steps. DNA stained with the red florescent dye will emit a red light (595 nm), very similar to Ethidium Bromide (EtBr).
Protocol :
1. Mix your samples with the safeload Loading Buffer in a 1:5 ratio. For example, add 1μl of the safeload Loading Buffer to 5 μl of DNA sample; or 2 μl of the safeload Loading Buffer to 10 μl of DNA sample.
2. Run the agarose gel and visualize under LED or UV light when ready. Take picture as needed normally.
