[SLA003] SAFELOAD 6X Agarose Loading Buffer
Cat. No. – SLA003
Package – 10ml
Storage – Cold (4-8℃) for 1 year, Avoid light
Description – red dye, non-carcinogen red fluorescent dye
Application – SAFELOAD Loading Buffer replace tixic Ethidium Bromide (EtBr)
Description :
SAFELOAD Loading Buffer replace toxic Ethidium Bromide (EtBr), a potent mutagen, for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose and polyacrylamide gel electrophoresis. SAFELOAD Loading Buffer contain red color tracking dye and a safe, non-carcinogen red fluorescent dye. Red dye is used for monitoring the agarose gel while it is running (approx 1 kb). During the gel electrophoresis, the agarose gel will reveal a red band. The gel can be directly visualized under UV light without any extra staining steps. DNA stained with the red florescent dye will emit a red light (595 nm), very similar to Ethidium Bromide (EtBr).
Protocol :
1. Mix your samples with the safeload Loading Buffer in a 1:5 ratio. For example, add 1μl of the safeload Loading Buffer to 5 μl of DNA sample; or 2 μl of the safeload Loading Buffer to 10 μl of DNA sample.
2. Run the agarose gel and visualize under LED or UV light when ready. Take picture as needed normally.
연관 상품
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[BH007] Hybridization Solution w/o formamide
더 보기Cat. No. – BH007
Package –500ml
Storage –RT
Description –40mM PIPES,pH 6.4, 0.1mM EDTA, pH 8.0, 0.4M NaCl
Application –Suitable for Southern, Northern and Dot Blot hybridization analysis, hybridization with oligonucleotides or other types of probes, and for hybridization screening of gene libraries
* For R&D use only
